• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC26 Polyclonal antibody

CDC26 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14125-1-AP

Synonyms

ANAPC12, APC12, C9orf17, CDC26

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CDC26 Polyclonal antibody (14125-1-AP)

    WB analysis using 14125-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14125-1-AP (CDC26 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDC26 Polyclonal antibody (14125-1-AP)

    IHC staining of mouse brain using 14125-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14125-1-AP (CDC26 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDC26 Polyclonal antibody (14125-1-AP)

    IHC staining of mouse brain using 14125-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14125-1-AP (CDC26 Antibody) at dilution of 1:50 (under 10x lens).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

14125-1-AP targets CDC26 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC26 fusion protein Ag5276 相同性解析による交差性が予測される生物種
Full Name cell division cycle 26 homolog (S. cerevisiae)
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC066300
Gene symbol CDC26
Gene ID (NCBI) 246184
RRIDAB_2878018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC26, also named as ANAPC12, APC12 and C9orf17, is a component of the anaphase promoting complex/cyclosome (APC/C). CDC26 can be detected as 14 kDa and ~18 kDa (PMID: 15060174 /PMID: 10222126).

Protocols

Product Specific Protocols
WB protocol for CDC26 antibody 14125-1-APDownload protocol
IHC protocol for CDC26 antibody 14125-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.

Authors - Kyungsoo Ha
View Article