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- KD/KO Validated
CDC27; APC3 Polyclonal antibody
CDC27; APC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10918-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
| IP | See 2 publications below |
Product Information
10918-1-AP targets CDC27; APC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDC27; APC3 fusion protein Ag1345 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle 27 homolog (S. cerevisiae) |
| Calculated molecular weight | 92 kDa |
| Observed molecular weight | 92 kDa |
| GenBank accession number | BC011656 |
| Gene symbol | CDC27 |
| Gene ID (NCBI) | 996 |
| RRID | AB_2075288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC27; APC3 antibody 10918-1-AP | Download protocol |
| IHC protocol for CDC27; APC3 antibody 10918-1-AP | Download protocol |
| IF protocol for CDC27; APC3 antibody 10918-1-AP | Download protocol |
| IP protocol for CDC27; APC3 antibody 10918-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma.
| ||
Oncotarget mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27. | ||
Cell Death Dis EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer. | ||
Cell Commun Signal APC/C-regulated CPT1C promotes tumor progression by upregulating the energy supply and accelerating the G1/S transition | ||
Cell Death Differ ABL1-mediated phosphorylation promotes FOXM1-related tumorigenicity by Increasing FOXM1 stability |
