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Target

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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

CDC37L1 Polyclonal antibody

CDC37L1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16293-1-AP

Synonyms

CDC37B, CDC37L1, HARC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC37L1 Polyclonal antibody (16293-1-AP)

    WB analysis using 16293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using CDC37L1 Polyclonal antibody (16293-1-AP)

    WB analysis of human kidney using 16293-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDC37L1 Polyclonal antibody (16293-1-AP)

    WB analysis of HeLa using 16293-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CDC37L1 Polyclonal antibody (16293-1-AP)

    IHC staining of human kidney using 16293-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CDC37L1 Polyclonal antibody (16293-1-AP)

    IHC staining of human kidney using 16293-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CDC37L1 Polyclonal antibody (16293-1-AP)

    IHC staining of human liver using 16293-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CDC37L1 Polyclonal antibody (16293-1-AP)

    IHC staining of human liver using 16293-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, human kidney tissue, Jurkat cells, LNCaP cells, MCF-7 cells, U2OS cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16293-1-AP targets CDC37L1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC37L1 fusion protein Ag9459 相同性解析による交差性が予測される生物種
Full Name cell division cycle 37 homolog (S. cerevisiae)-like 1
Calculated molecular weight 337 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC014133
Gene symbol CDC37L1
Gene ID (NCBI) 55664
RRIDAB_2244711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CDC37L1 antibody 16293-1-APDownload protocol
IHC protocol for CDC37L1 antibody 16293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

J Cancer

CDC37L1 acts as a suppressor of migration and proliferation in gastric cancer by down-regulating CDK6.

Authors - Li Li
  • KD Validated
View Article
humanWB,IHC

Cell Death Discov

miR-15a and miR-20b sensitize hepatocellular carcinoma cells to sorafenib through repressing CDC37L1 and consequent PPIA downregulation.

Authors - Li Li
View Article