• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC42 Monoclonal antibody

CDC42 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G5E8

Cat no : 67212-1-Ig

Synonyms

CDC42, CDC42Hs, G25K, G25K GTP binding protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CDC42 Monoclonal antibody (67212-1-Ig)

    WB analysis using 67212-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CDC42 Monoclonal antibody (67212-1-Ig)

    WB analysis using 67212-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CDC42 Monoclonal antibody (67212-1-Ig)

    WB analysis of mouse cerebellum using 67212-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CDC42 Monoclonal antibody (67212-1-Ig)

    WB analysis using 67212-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHeLa cells, rat brain tissue, mouse cerebellum tissue, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67212-1-Ig targets CDC42 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen CDC42 fusion protein Ag28550 相同性解析による交差性が予測される生物種
Full Name cell division cycle 42 (GTP binding protein, 25kDa)
Calculated molecular weight 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC002711
Gene symbol CDC42
Gene ID (NCBI) 998
RRIDAB_2918484
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking

Protocols

Product Specific Protocols
WB protocol for CDC42 antibody 67212-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols