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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC42EP4 Polyclonal antibody

CDC42EP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19262-1-AP

Synonyms

CEP4, Cdc42 effector protein 4, BORG4, Binder of Rho GTPases 4

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CDC42EP4 Polyclonal antibody (19262-1-AP)

    WB analysis of K-562 using 19262-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CDC42EP4 Polyclonal antibody (19262-1-AP)

    WB analysis of A549 using 19262-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CDC42EP4 Polyclonal antibody (19262-1-AP)

    WB analysis of mouse lung using 19262-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CDC42EP4 Polyclonal antibody (19262-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 19262-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19262-1-AP (CDC42EP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CDC42EP4 Polyclonal antibody (19262-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 19262-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19262-1-AP (CDC42EP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, A549 cells, mouse lung tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19262-1-AP targets CDC42EP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC42EP4 fusion protein Ag13594 相同性解析による交差性が予測される生物種
Full Name CDC42 effector protein (Rho GTPase binding) 4
Calculated molecular weight 38 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC010451
Gene symbol CDC42EP4
Gene ID (NCBI) 23580
RRIDAB_10598005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC42EP4, or Cell division cycle 42 effector protein 4, is a member of the CDC42-binding protein family. This protein interacts with Rho family GTPases and plays a role in regulating the organization of the actin cytoskeleton. It has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. Overexpression of CDC42EP4 in fibroblasts can induce pseudopodia formation, suggesting its involvement in actin filament assembly and cell shape control. CDC42EP4 is also implicated in cancer progression, particularly in prostate cancer. Furthermore, CDC42EP4 has been implicated in the regulation of glutamate transporters, which are crucial for maintaining glutamate homeostasis in the nervous system. It has been suggested that CDC42EP4 may interact with septins, a family of polymerizing GTPases that constitute the membrane skeleton, and this interaction could potentially influence the localization and activity of glutamate transporters.

Protocols

Product Specific Protocols
WB protocol for CDC42EP4 antibody 19262-1-APDownload protocol
IHC protocol for CDC42EP4 antibody 19262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols