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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC6 Monoclonal antibody (66021-1-Ig)

    WB analysis using 66021-1-Ig (same clone as 66021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using CDC6 Monoclonal antibody (66021-1-Ig)

    WB analysis of ROS1728 using 66021-1-Ig (same clone as 66021-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CDC6 Monoclonal antibody (66021-1-Ig)

    WB analysis of NIH/3T3 using 66021-1-Ig (same clone as 66021-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CDC6 Monoclonal antibody (66021-1-Ig)

    WB analysis of HeLa using 66021-1-Ig (same clone as 66021-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDC6 Monoclonal antibody (66021-1-Ig)

    IF Staining of HeLa using 66021-1-Ig (same clone as 66021-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CDC6 antibody (66021-1-Ig, Clone: 6F12D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

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CDC6 Monoclonal antibody, PBS Only

CDC6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66021-1-PBS
Clone No.6F12D2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CDC6-related protein, CDC6 related protein, Cdc18-related protein, CDC18L, Cdc18 related protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66021-1-PBS targets CDC6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDC6 fusion protein Ag17958 相同性解析による交差性が予測される生物種
Full Name cell division cycle 6 homolog (S. cerevisiae)
Calculated molecular weight 560 aa, 63 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC025232
Gene Symbol CDC6
Gene ID (NCBI) 990
RRIDAB_2881493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99741
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.