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  • Featured Product
  • KD/KO Validated

Cdc7 Kinase Polyclonal antibody

Cdc7 Kinase Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17980-1-AP

Synonyms

EC:2.7.11.1, Cell division cycle 7-related protein kinase, CDC7-related kinase, CDC7L1, CDC7 related kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using Cdc7 Kinase Polyclonal antibody (17980-1-AP)

    WB analysis of SH-SY5Y using 17980-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17980-1-AP (Cdc7 Kinase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cdc7 Kinase Polyclonal antibody (17980-1-AP)

    WB analysis of HeLa using 17980-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17980-1-AP (Cdc7 Kinase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cdc7 Kinase Polyclonal antibody (17980-1-AP)

    IF Staining of HepG2 using 17980-1-AP

    Immunofluorescent analysis of HepG2 cells, using CDC7 antibody 17980-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17980-1-AP targets Cdc7 Kinase in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cdc7 Kinase fusion protein Ag12384 相同性解析による交差性が予測される生物種
Full Name cell division cycle 7 homolog (S. cerevisiae)
Calculated molecular weight 574 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC111044
Gene symbol CDC7
Gene ID (NCBI) 8317
RRIDAB_2291604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cdc7 Kinase antibody 17980-1-APDownload protocol
IF protocol for Cdc7 Kinase antibody 17980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer.

Authors - Yiming He
View Article
humanWB

Cell Death Discov

Identifying CDC7 as a synergistic target of chemotherapy in resistant small-cell lung cancer via CRISPR/Cas9 screening

Authors - Ling Deng
  • KD Validated
View Article