• Featured Product
  • KD/KO Validated

CDCA4 Polyclonal antibody

CDCA4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11625-1-AP

Synonyms

CDCA4, HEPP, SEI 3/HEPP


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11625-1-AP targets CDCA4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDCA4 fusion protein Ag2214 相同性解析による交差性が予測される生物種
Full Name cell division cycle associated 4
Calculated molecular weight 241 aa, 26 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC025263
Gene symbol CDCA4
Gene ID (NCBI) 55038
RRIDAB_2260414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIP-Br proteins are a set of proteins with the potential to function in both transcriptional control and cell cycle regulation [PMID:18572021]. CECA4 is a member of TRIP-br family, based on its homologous sequence motifs including SERTA and a PHD-bromo-binding site which interacts with a series of bromodomain containing transcriptional cofactors[PMID:17141982]. When activated by E2F, CDCA4 consequently functions as a suppressor of E2F-responsive promoters to participate the regulation of cell proliferation through the E2F/pRb pathway [PMID:16098148].

Protocols

Product Specific Protocols
WB protocol for CDCA4 antibody 11625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cancer Cell

The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia.

Authors - Ulf Klein
  • KD Validated
humanIF

Sci Rep

Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.

Authors - Honghai Xia
humanWB,CoIP

Cancer Cell Int

CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.

Authors - Chenxin Xu
  • KD Validated
humanWB,IP

Thorac Cancer

CDCA4 interacts with IGF2BP1 to regulate lung adenocarcinoma proliferation via the PI3K/AKT pathway

Authors - Sitong Feng
  • KD Validated
human,mouseWB

Gene

ALKBH5 promotes the development of lung adenocarcinoma by regulating the polarization of M2 macrophages through CDCA4

Authors - Jianlong Tan
  • KD Validated
humanWB

Front Mol Biosci

Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients

Authors - Liping Shi