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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDCA5 Monoclonal antibody (67418-1-Ig)

    WB analysis using 67418-1-Ig (same clone as 67418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67418-1-Ig (CDCA5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDCA5 Monoclonal antibody (67418-1-Ig)

    IHC staining of human lung cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDCA5 Monoclonal antibody (67418-1-Ig)

    IHC staining of human lung cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDCA5 Monoclonal antibody (67418-1-Ig)

    IHC staining of human colon cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDCA5 Monoclonal antibody (67418-1-Ig)

    IHC staining of human colon cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDCA5 Monoclonal antibody (67418-1-Ig)

    IHC staining of human colon cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDCA5 Monoclonal antibody (67418-1-Ig)

    IHC staining of human colon cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDCA5 Monoclonal antibody (67418-1-Ig)

    IF Staining of HeLa using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDCA5 antibody (67418-1-Ig, Clone: 2G1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

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CDCA5 Monoclonal antibody, PBS Only

CDCA5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67418-1-PBS
Clone No.2G1D2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CDCA5, p35, SORORIN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67418-1-PBS targets CDCA5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDCA5 fusion protein Ag28958 相同性解析による交差性が予測される生物種
Full Name cell division cycle associated 5
Calculated molecular weight 27 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC011000
Gene Symbol CDCA5
Gene ID (NCBI) 113130
RRIDAB_2882658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96FF9
Storage Buffer PBS only
Storage ConditionsStore at -80°C.