Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, Raji cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
15249-1-AP targets CDCA7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDCA7 fusion protein Ag4297 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle associated 7 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 40-43 kDa |
| GenBank accession number | BC027966 |
| Gene Symbol | CDCA7 |
| Gene ID (NCBI) | 83879 |
| RRID | AB_2878119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BWT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDCA7, also named as JP01, participates in MYC-mediated cell transformation and apoptosis. It plays a role as transcriptional regulator.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| IHC protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| IF protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| IP protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS | ||
Sci Adv CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses | ||
Proc Natl Acad Sci U S A HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex defective in ICF syndrome. | ||
J Exp Med Loss of ZBTB24 impairs nonhomologous end-joining and class-switch recombination in patients with ICF syndrome. | ||
Hum Mol Genet Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals. | ||
Bioorg Chem Novel isatin-based hybrids as potential anti-rheumatoid arthritis drug candidates: Synthesis and biological evaluation |