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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDCA7L Recombinant antibody

CDCA7L Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240639A3

Cat no : 83682-4-RR

Synonyms

R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDCA7L Recombinant antibody (83682-4-RR)

    WB analysis using 83682-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83682-4-RR (CDCA7L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using CDCA7L Recombinant antibody (83682-4-RR)

    FC experiment of A431 using 83682-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CDCA7L Recombinant antibody (83682-4-RR, Clone:240639A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83682-4-RR

    Affinity and Kinetic Characterization of 83682-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83682-4-RR against Human CDCA7L were performed. The affinity constant is 7.75 nM.

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Tested Applications

Positive WB detected inA549 cells, HEK-293T cells, HeLa cells, K-562 cells, MCF-7 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83682-4-RR targets CDCA7L in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDCA7L fusion protein Ag10944 相同性解析による交差性が予測される生物種
Full Name cell division cycle associated 7-like
Calculated molecular weight 454 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC009352
Gene symbol CDCA7L
Gene ID (NCBI) 55536
RRIDAB_3671283
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cell division cycle-associated 7-like protein (CDCA7L) is also known as HR1 and JPO2 and belongs to the JPO protein family. CDCA7L is identified as a target gene of c-Myc and is frequently dysregulated in multiple cancers. CDCA7L can complement the c-Myc transformation-defective mutant W135E and potentiate Myc-mediated transformation (PMID: 24141559, PMID: 37140023). CDCA7L translocates from cytoplasm to nucleus under dexamethasone induction. CDCA7L is widely expressed. CDCA7L has 4 isoforms produced by alternative splicing (refer to UniProt).

Protocols

Product Specific Protocols
WB protocol for CDCA7L antibody 83682-4-RRDownload protocol
FC protocol for CDCA7L antibody 83682-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols