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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDCP1 Polyclonal antibody

CDCP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12754-1-AP

Synonyms

Transmembrane and associated with src kinases, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDCP1 Polyclonal antibody (12754-1-AP)

    WB analysis using 12754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12754-1-AP (CDCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CDCP1 Polyclonal antibody (12754-1-AP)

    WB analysis of A549 using 12754-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12754-1-AP (CDCP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CDCP1 Polyclonal antibody (12754-1-AP)

    IHC staining of human skin cancer using 12754-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12754-1-AP (CDCP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CDCP1 Polyclonal antibody (12754-1-AP)

    IHC staining of human skin cancer using 12754-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12754-1-AP (CDCP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, LNCaP cells, PC-3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12754-1-AP targets CDCP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDCP1 fusion protein Ag3439 相同性解析による交差性が予測される生物種
Full Name CUB domain containing protein 1
Calculated molecular weight 836 aa, 93 kDa
Observed molecular weight 72 kDa, 130 kDa
GenBank accession numberBC021099
Gene symbol CDCP1
Gene ID (NCBI) 64866
RRIDAB_2078799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDCP1, also called CD318, TRASK, and SIMA 135, is a 135-140 kDa transmembrane protein containing three CUB domains within the extracellular region and a hexalysine stretch within the cytoplasmic region. A soluble form with MW 80 kDa (p80) is cleaved at an unidentified site on the N-terminal side. CDCP1 is a novel marker for hematopoietic stem cells.

Protocols

Product Specific Protocols
WB protocol for CDCP1 antibody 12754-1-APDownload protocol
IHC protocol for CDCP1 antibody 12754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Theranostics

Radiation-induced small extracellular vesicles as "carriages" promote tumor antigen release and trigger antitumor immunity.

Authors - Wanzun Lin
View Article
humanWB

Aging (Albany NY)

The emerging role of the MiR-1272-ADAM9-CDCP1 signaling pathway in the progression of glioma.

Authors - Fei Geng
View Article