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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using Cadherin-11 Polyclonal antibody (12028-1-AP)

    WB analysis of PC-3 using 12028-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12028-1-AP (Cadherin-11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Cadherin-11 Polyclonal antibody

Cadherin-11 Polyclonal Antibody for WB, ELISA
Cat No. 12028-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CDH11, CAD11, Cadherin 11, CDHOB, OB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12028-1-AP targets Cadherin-11 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cadherin-11 fusion protein Ag2659 相同性解析による交差性が予測される生物種
Full Name cadherin 11, type 2, OB-cadherin (osteoblast)
Calculated molecular weight 796 aa, 88 kDa
Observed molecular weight120 kDa
GenBank accession numberBC013609
Gene Symbol CDH11
Gene ID (NCBI) 1009
RRIDAB_3085386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55287
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-11 (CDH11), also known osteoblast cadherin (OB-cadherin) or OSF-4, was identified initially in a mouse osteoblastic cell line and human osteosarcoma (PMID: 8163513). Cadherin-11 is a single pass transmembrane protein consisting of an extracellular domain which contains five repeat sequences (EC1-EC5), a transmembrane segment and a cytoplasmic domain which links catenins to the actin and microtubule cytoskeleton (PMID: 8163513; 33442417). It has been shown that cadherin-11 plays important roles in the occurrence and development of a lot of diseases, arthritis, cancer, aortic calcification disease, dermatosis (PMID: 33442417).

Protocols

Product Specific Protocols
WB protocol for Cadherin-11 antibody 12028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols