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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VE-cadherin/CD144 Polyclonal antibody

VE-cadherin/CD144 Polyclonal Antibody for WB, IHC, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 27956-1-AP

Synonyms

VE-cadherin, Vascular endothelial cadherin, CDH5, CD144, Cadherin 5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using VE-cadherin Polyclonal antibody (27956-1-AP)

    WB analysis using 27956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27956-1-AP (VE-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VE-cadherin Polyclonal antibody (27956-1-AP)

    IHC staining of human placenta using 27956-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27956-1-AP (VE-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using VE-cadherin Polyclonal antibody (27956-1-AP)

    IHC staining of human placenta using 27956-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27956-1-AP (VE-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VE-cadherin Polyclonal antibody (27956-1-AP)

    IHC staining of human lung cancer using 27956-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27956-1-AP (VE-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VE-cadherin Polyclonal antibody (27956-1-AP)

    IHC staining of human lung cancer using 27956-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27956-1-AP (VE-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VE-cadherin Polyclonal antibody (27956-1-AP)

    IHC staining of human breast cancer using 27956-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27956-1-AP (CDH5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VE-cadherin Polyclonal antibody (27956-1-AP)

    IHC staining of human breast cancer using 27956-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27956-1-AP (CDH5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HUVEC cells using VE-cadherin Polyclonal antibody (27956-1-AP)

    FC experiment of HUVEC using 27956-1-AP

    1X10^6 HUVEC cells were surface stained with 0.4 ug Anti-Human VE-cadherin (27956-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody. Cells were not fixed.

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Tested Applications

Positive WB detected inHUVEC cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27956-1-AP targets VE-cadherin/CD144 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VE-cadherin/CD144 fusion protein Ag27487 相同性解析による交差性が予測される生物種
Full Name cadherin 5, type 2 (vascular endothelium)
Calculated molecular weight 88 kDa
Observed molecular weight120-140 kDa
GenBank accession numberNM_001795
Gene symbol VE-cadherin
Gene ID (NCBI) 1003
RRIDAB_2918136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

Protocols

Product Specific Protocols
WB protocol for VE-cadherin/CD144 antibody 27956-1-APDownload protocol
IHC protocol for VE-cadherin/CD144 antibody 27956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Commun

FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment

Authors - Zongchao Sun
View Article
humanIF

Bioengineering (Basel)

Organotypic 3D Co-Culture of Human Pleura as a Novel In Vitro Model of Staphylococcus aureus Infection and Biofilm Development

Authors - Olga Kurow
View Article
WB

World J Diabetes

Tongxinluo promotes endothelium-dependent arteriogenesis to attenuate diabetic peripheral arterial disease

Authors - Jiao-Jiao Gu
View Article
WB

Front Cell Infect Microbiol

Analysis of miRNAs Involved in Mouse Heart Injury Upon Coxsackievirus A2 Infection.

Authors - Zhaoke Wu
View Article
humanIF

Bioengineering (Basel)

A Transwell-Based Vascularized Model to Investigate the Effect of Interstitial Flow on Vasculogenesis

Authors - Pengwei Deng
View Article
humanIF

J Hypertens

Short-chain acyl-CoA dehydrogenase is a potential target for the treatment of vascular remodelling

Authors - Xiaoyi Zhong
View Article