CDK1 Monoclonal antibody
CDK1 Monoclonal Antibody for WB, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2A11E4
Cat no : 67575-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 13 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
67575-1-Ig targets CDK1 in WB, IHC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CDK1 fusion protein Ag29018 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle 2, G1 to S and G2 to M |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC014563 |
| Gene symbol | CDK1 |
| Gene ID (NCBI) | 983 |
| RRID | AB_2882788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK1 antibody 67575-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17 | ||
Mol Carcinog YAP/TEAD4-induced KIF4A contributes to the progression and worse prognosis of esophageal squamous cell carcinoma. | ||
Onco Targets Ther Sinomenine Inhibits the Growth of Ovarian Cancer Cells Through the Suppression of Mitosis by Down-Regulating the Expression and the Activity of CDK1. | ||
Chin Med A network pharmacology approach to reveal the pharmacological targets and biological mechanism of compound kushen injection for treating pancreatic cancer based on WGCNA and in vitro experiment validation | ||
BMC Cardiovasc Disord Role of ferroptosis-related genes in coronary atherosclerosis and identification of key genes: integration of bioinformatics analysis and experimental validation | ||
ACS Pharmacol Transl Sci Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening |
