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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK10 Polyclonal antibody (30061-1-AP)

    WB analysis using 30061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30061-1-AP (CDK10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CDK10 Polyclonal antibody (30061-1-AP)

    IF Staining of hTERT-RPE1 using 30061-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CDK10 antibody (30061-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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CDK10 Polyclonal antibody

CDK10 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30061-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Serine/threonine-protein kinase PISSLRE, EC:2.7.11.22, Cyclin-dependent kinase 10, Cyclin dependent kinase 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, K-562 cells, THP-1 cells, U2OS cells, C2C12 cells
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30061-1-AP targets CDK10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK10 fusion protein Ag30365 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 10
Calculated molecular weight 283 aa, 32 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC017342
Gene Symbol CDK10
Gene ID (NCBI) 8558
RRIDAB_2935507
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15131
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin-dependent kinase 10 (CDK10) is a Cdc2-related kinase that was discovered based on its homology to the Cdc2 PSTA1RE amino acid domain (PMID: 8208557). CDK10 plays a pivotal role in the regulation of fundamental cellular processes, including cell proliferation, transcription regulation, and cell cycle regulation. It partners with cyclin M to phosphorylate substrates such as ETS2 and PKN2 in order to modulate cellular growth. Initial reports have indicated that CDK10 may act as a tumor suppressor in breast cancer (PMID: 26392360). Additional studies have demonstrated tumor suppressive and oncogenic roles for CDK10 in malignancies such as hepatobiliary cancers, gastric cancer, glioma, nasopharyngeal carcinoma, and colorectal cancer(PMID: 22326270, PMID: 29512714, PMID: 23740091, PMID: 29845196)

Protocols

Product Specific Protocols
WB protocol for CDK10 antibody 30061-1-APDownload protocol
IF protocol for CDK10 antibody 30061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols