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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK2 Recombinant antibody

CDK2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240580G11

Cat no : 83635-2-RR

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK2 Recombinant antibody (83635-2-RR)

    WB analysis using 83635-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83635-2-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using CDK2 Recombinant antibody (83635-2-RR)

    IHC staining of human ovarian cancer using 83635-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83635-2-RR (CDK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovarian cancer using CDK2 Recombinant antibody (83635-2-RR)

    IHC staining of human ovarian cancer using 83635-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83635-2-RR (CDK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDK2 Recombinant antibody (83635-2-RR)

    IF Staining of HepG2 using 83635-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (83635-2-RR, Clone: 240580G11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using CDK2 Recombinant antibody (83635-2-RR)

    FC experiment of HeLa using 83635-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-2-RR, Clone:240580G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83635-2-RR

    Affinity and Kinetic Characterization of 83635-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83635-2-RR against Human CDK2 were performed. The affinity constant is 3.23 nM.

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Tested Applications

Positive WB detected inMCF-7 cells, K-562 cells, HEK-293 cells, HeLa cells, HepG2 cells, C6 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83635-2-RR is CDK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK2 fusion protein Ag17133 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 2
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC003065
Gene symbol CDK2
Gene ID (NCBI) 1017
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CDK2 antibody 83635-2-RRDownload protocol
IHC protocol for CDK2 antibody 83635-2-RRDownload protocol
IF protocol for CDK2 antibody 83635-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols