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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK2 Recombinant antibody, PBS Only

CDK2 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240580A11

Cat no : 83635-4-PBS

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK2 Recombinant antibody (83635-4-RR)

    WB analysis using 83635-4-RR (same clone as 83635-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83635-4-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83635-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CDK2 Recombinant antibody (83635-4-RR)

    FC experiment of HeLa using 83635-4-RR (same clone as 83635-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-4-RR, Clone:240580A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83635-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83635-4-RR

    Affinity and Kinetic Characterization of 83635-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83635-4-RR against Human CDK2 were performed. The affinity constant is 8.32 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83635-4-PBS is CDK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK2 fusion protein Ag17133 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 2
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC003065
Gene symbol CDK2
Gene ID (NCBI) 1017
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.