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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK5R1 Monoclonal antibody (67503-1-Ig)

    WB analysis using 67503-1-Ig (same clone as 67503-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CDK5R1 Monoclonal antibody (67503-1-Ig)

    WB analysis of mouse cerebellum using 67503-1-Ig (same clone as 67503-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using CDK5R1 Monoclonal antibody (67503-1-Ig)

    WB analysis of pig brain using 67503-1-Ig (same clone as 67503-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using CDK5R1 Monoclonal antibody (67503-1-Ig)

    WB analysis of rat brain using 67503-1-Ig (same clone as 67503-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CDK5R1 Monoclonal antibody (67503-1-Ig)

    WB analysis of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDK5R1 Monoclonal antibody (67503-1-Ig)

    IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDK5R1 Monoclonal antibody (67503-1-Ig)

    IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CDK5R1 Monoclonal antibody (67503-1-Ig)

    IHC staining of rat brain using 67503-1-Ig (same clone as 67503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CDK5R1 Monoclonal antibody (67503-1-Ig)

    IHC staining of rat brain using 67503-1-Ig (same clone as 67503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

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CDK5R1 Monoclonal antibody, PBS Only

CDK5R1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67503-1-PBS
Clone No.1A3A5

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67503-1-PBS targets CDK5R1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDK5R1 fusion protein Ag13152 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 5, regulatory subunit 1 (p35)
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC020580
Gene Symbol CDK5R1
Gene ID (NCBI) 8851
RRIDAB_2882727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15078
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.