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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK5R2/p39 Polyclonal antibody

CDK5R2/p39 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27058-1-AP

Synonyms

CDK5 activator 2, CDK5R2, CDK5R2/p39, NCK5AI, P39, p39I, p39nck5ai

Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using CDK5R2/p39 Polyclonal antibody (27058-1-AP)

    WB analysis of Neuro-2a using 27058-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27058-1-AP (CDK5R2/p39 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of various lysates using CDK5R2/p39 Polyclonal antibody (27058-1-AP)

    WB analysis using 27058-1-AP

    various lysates were subjected to SDS PAGE followed by western blot with 27058-1-AP (CDK5R2/p39 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDK5R2/p39 Polyclonal antibody (27058-1-AP)

    IHC staining of mouse brain using 27058-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27058-1-AP (CDK5R2/p39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27058-1-AP targets CDK5R2/p39 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK5R2/p39 fusion protein Ag25834 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 5, regulatory subunit 2 (p39)
Observed molecular weight 39 kDa
GenBank accession numberBC041771
Gene symbol CDK5R2/p39
Gene ID (NCBI) 8941
RRIDAB_2880735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK5R2 is a neuron-specific activator of CDK5 kinase. It associates with CDK5 to form an active kinase. This protein and neuron-specific CDK5 activator CDK5R1/p39NCK5A both share limited similarity to cyclins, and thus may define a distinct family of cyclin-dependent kinase activating proteins.

Protocols

Product Specific Protocols
WB protocol for CDK5R2/p39 antibody 27058-1-APDownload protocol
IHC protocol for CDK5R2/p39 antibody 27058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

m1A regulator-mediated methylation modification patterns correlated with autophagy to predict the prognosis of hepatocellular carcinoma

Authors - Yingmin Wu
View Article