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  • Featured Product
  • KD/KO Validated

CDK5RAP1 Polyclonal antibody

CDK5RAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14740-1-AP

Synonyms

C20orf34, C42, CDK5RAP1, CDK5RAP1.3, CDK5RAP1.4, CGI 05, HSPC167

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK5RAP1 Polyclonal antibody (14740-1-AP)

    WB analysis using 14740-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using CDK5RAP1 Polyclonal antibody (14740-1-AP)

    WB analysis of human heart using 14740-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CDK5RAP1 Polyclonal antibody (14740-1-AP)

    WB analysis of mouse brain using 14740-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CDK5RAP1 Polyclonal antibody (14740-1-AP)

    IHC staining of human pancreas using 14740-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDK5RAP1 Polyclonal antibody (14740-1-AP)

    IHC staining of mouse brain using 14740-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using CDK5RAP1 Polyclonal antibody (14740-1-AP)

    IHC staining of rat brain using 14740-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using CDK5RAP1 Polyclonal antibody (14740-1-AP)

    IHC staining of rat brain using 14740-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CDK5RAP1 Polyclonal antibody (14740-1-AP)

    IHC staining of human pancreas using 14740-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDK5RAP1 Polyclonal antibody (14740-1-AP)

    IHC staining of mouse brain using 14740-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inC6 cells, human heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14740-1-AP targets CDK5RAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK5RAP1 fusion protein Ag6409 相同性解析による交差性が予測される生物種
Full Name CDK5 regulatory subunit associated protein 1
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC050706
Gene symbol CDK5RAP1
Gene ID (NCBI) 51654
RRIDAB_2076843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CDK5RAP1 antibody 14740-1-APDownload protocol
IHC protocol for CDK5RAP1 antibody 14740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

METTL17 is an Fe-S cluster checkpoint for mitochondrial translation

Authors - Tslil Ast
  • KD Validated
View Article