Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
20061-1-AP targets CDK5RAP2-Specific in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | CDK5 regulatory subunit associated protein 2 |
| Calculated molecular weight | 215 kDa |
| GenBank accession number | NM_001011649 |
| Gene Symbol | CDK5RAP2 |
| Gene ID (NCBI) | 55755 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96SN8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDK5RAP2, also named as CEP215 and KIAA1633, belongs to the methylthiotransferase family and MiaB subfamily. This protein binds to p25NCK5A and therefore may be involved in neuronal differentiation. It may also be a substrate of neuronal CDC2-like kinase. CDK5RAP2 specifically inhibits CDK5 activation by CDK5R1. Defects in CDK5RAP2 are the cause of primary microcephaly autosomal recessive type 3 (MCPH3). Multiple isoforms generated through co- and/or posttranslational modifications or degradation may exist as scientists have described bands of 70, 90, 105, 200, and 230 kDa in mice depending on the age and organ investigated as well as the anti-Cdk5rap2 antibody applied or 210kd in total cell lysates of some human canser cell lines. (PUMID: 21327915, 20471352 and 18042621). The antibody is specific to CDK5RAP2.
Publications
| Species | Application | Title |
|---|---|---|
PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. |