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CDK5RAP3 Polyclonal antibody
CDK5RAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 11007-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, HEK-293T cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human stomach tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
11007-1-AP targets CDK5RAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDK5RAP3 fusion protein Ag1474 相同性解析による交差性が予測される生物種 |
| Full Name | CDK5 regulatory subunit associated protein 3 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC009957 |
| Gene symbol | CDK5RAP3 |
| Gene ID (NCBI) | 80279 |
| RRID | AB_2076869 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK5RAP3 antibody 11007-1-AP | Download protocol |
| IHC protocol for CDK5RAP3 antibody 11007-1-AP | Download protocol |
| IF protocol for CDK5RAP3 antibody 11007-1-AP | Download protocol |
| FC protocol for CDK5RAP3 antibody 11007-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury.
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