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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK7 Polyclonal antibody

CDK7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27027-1-AP

Synonyms

CDKN7, CDK-activating kinase 1, CDK activating kinase, CAK1, CAK

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CDK7 Polyclonal antibody (27027-1-AP)

    WB analysis of HepG2 using 27027-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CDK7 Polyclonal antibody (27027-1-AP)

    WB analysis of HepG2 using 27027-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse embryo tissue using CDK7 Polyclonal antibody (27027-1-AP)

    WB analysis of mouse embryo using 27027-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CDK7 Polyclonal antibody (27027-1-AP)

    WB analysis of MCF-7 using 27027-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK7 Polyclonal antibody (27027-1-AP)

    IHC staining of human breast cancer using 27027-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK7 Polyclonal antibody (27027-1-AP)

    IHC staining of human breast cancer using 27027-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDK7 Polyclonal antibody (27027-1-AP)

    IF Staining of HepG2 using 27027-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK7 antibody (27027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, mouse embryo tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

27027-1-AP targets CDK7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK7 fusion protein Ag25726 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 7
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC005298
Gene symbol CDK7
Gene ID (NCBI) 1022
RRIDAB_2880728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CDK7 antibody 27027-1-APDownload protocol
IHC protocol for CDK7 antibody 27027-1-APDownload protocol
IF protocol for CDK7 antibody 27027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Nano

Nanomaterial-Facilitated Cyclin-Dependent Kinase 7 Inhibition Suppresses Gallbladder Cancer Progression via Targeting Transcriptional Addiction.

Authors - Chen-Song Huang
View Article
mouseWB

Genes Dev

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Authors - Zhe Yang
View Article
humanWB

Front Oncol

Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach

Authors - Xiaobin Fei
View Article
humanWB

Cell Signal

TAGLN2 targeted control of ARPC5-mediated activation of the MEK/ERK signaling pathway influences the proliferation, invasion, and metastasis of pancreatic cancer cells

Authors - Yongjia Gao
View Article