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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK7 Recombinant antibody (83976-3-RR)

    WB analysis using 83976-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83976-3-RR (CDK7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDK7 Recombinant antibody (83976-3-RR)

    IF Staining of HepG2 using 83976-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK7 antibody (83976-3-RR, Clone: 240962F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83976-3-RR

    Affinity and Kinetic Characterization of 83976-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83976-3-RR against Human CDK7 were performed. The affinity constant is 28.7 nM.

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CDK7 Recombinant antibody

CDK7 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83976-3-RR
Clone No.240962F9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CDK-activating kinase 1, CDK activating kinase, CAK1, CAK, 39 kDa protein kinase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83976-3-RR targets CDK7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK7 fusion protein Ag25737 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 7
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC005298
Gene Symbol CDK7
Gene ID (NCBI) 1022
RRIDAB_3671551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50613
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CDK7 antibody 83976-3-RRDownload protocol
IF protocol for CDK7 antibody 83976-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols