• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK9 Polyclonal antibody, PBS Only

CDK9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11705-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CDK9 Polyclonal antibody (11705-1-AP)

    WB analysis using 11705-1-AP (same clone as 11705-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11705-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CDK9 Polyclonal antibody (11705-1-AP)

    WB analysis of Jurkat using 11705-1-AP (same clone as 11705-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11705-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CDK9 Polyclonal antibody (11705-1-AP)

    WB analysis of human placenta using 11705-1-AP (same clone as 11705-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11705-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CDK9 Polyclonal antibody (11705-1-AP)

    IHC staining of human gliomas using 11705-1-AP (same clone as 11705-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11705-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CDK9 Polyclonal antibody (11705-1-AP)

    IHC staining of human gliomas using 11705-1-AP (same clone as 11705-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11705-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDK9 Polyclonal antibody (11705-1-AP)

    IHC staining of human lung cancer using 11705-1-AP (same clone as 11705-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11705-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDK9 Polyclonal antibody (11705-1-AP)

    IHC staining of human lung cancer using 11705-1-AP (same clone as 11705-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11705-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CDK9 Polyclonal antibody (11705-1-AP)

    IF Staining of A431 using 11705-1-AP (same clone as 11705-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDK9 antibody (11705-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11705-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK9 Polyclonal antibody (11705-1-AP)

    IF Staining of HeLa using 11705-1-AP (same clone as 11705-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (11705-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11705-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11705-1-PBS targets CDK9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK9 fusion protein Ag2318 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 9
Calculated molecular weight 372 aa, 43 kDa
Observed molecular weight 38-42 kDa, 55 kDa
GenBank accession numberBC001968
Gene symbol CDK9
Gene ID (NCBI) 1025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).