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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK9 Monoclonal antibody

CDK9 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G6B3

Cat no : 67256-1-Ig

Synonyms

C 2k, CDC2L4, CDK9, Cell division protein kinase 9, CTK1, cyclin dependent kinase 9, PITALRE, TAK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK9 Monoclonal antibody (67256-1-Ig)

    WB analysis using 67256-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67256-1-Ig (CDK9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK9 Monoclonal antibody (67256-1-Ig)

    IF Staining of HeLa using 67256-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (67256-1-Ig, Clone: 1G6B3 ) at dilution of 1:1000 and Multi-rAbCoraLite Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

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Tested Applications

Positive WB detected inHeLa cells, T-47D cells, HCT 116 cells, A431 cells, MOLT-4 cells, TF-1 cells, HSC-T6 cells, ROS1728 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67256-1-Ig targets CDK9 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDK9 fusion protein Ag29178 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 9
Calculated molecular weight 372 aa, 43 kDa
Observed molecular weight38-42 kDa, 55 kDa
GenBank accession numberBC001968
Gene symbol CDK9
Gene ID (NCBI) 1025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).

Protocols

Product Specific Protocols
WB protocol for CDK9 antibody 67256-1-IgDownload protocol
IF protocol for CDK9 antibody 67256-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols