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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

NKIAMRE Polyclonal antibody

NKIAMRE Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13888-1-AP

Synonyms

CDKL3, cyclin dependent kinase like 3, NKIAMRE

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NKIAMRE Polyclonal antibody (13888-1-AP)

    WB analysis of HeLa using 13888-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13888-1-AP (NKIAMRE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NKIAMRE Polyclonal antibody (13888-1-AP)

    WB analysis of HeLa using 13888-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13888-1-AP (NKIAMRE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NKIAMRE Polyclonal antibody (13888-1-AP)

    IHC staining of human gliomas using 13888-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13888-1-AP (NKIAMRE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NKIAMRE Polyclonal antibody (13888-1-AP)

    IHC staining of human gliomas using 13888-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13888-1-AP (NKIAMRE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13888-1-AP targets NKIAMRE in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKIAMRE fusion protein Ag4812 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase-like 3
Calculated molecular weight 592 aa, 68 kDa
Observed molecular weight 52-60 kDa
GenBank accession numberBC041799
Gene symbol NKIAMRE
Gene ID (NCBI) 51265
RRIDAB_2077463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NKIAMRE is also named as CDKL3(Cyclin-dependent kinase-like 3) and belongs to the protein kinase superfamily.This protein contains the MAPK-like TDY motif for threonine and tyrosine phosphorylation, as well as the putative cyclin-binding motif(PMID:10463609). It has 2 isoforms produced by alternative splicing with the molecular mass of 52 kDa and 68 kDa.

Protocols

Product Specific Protocols
WB protocol for NKIAMRE antibody 13888-1-APDownload protocol
IHC protocol for NKIAMRE antibody 13888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

CDKL3 Targets ATG5 to Promote Carcinogenesis of Esophageal Squamous Cell Carcinoma.

Authors - Suna Zhou
View Article
humanWB,IHC

Front Physiol

Curcumol Exerts Anticancer Effect in Cholangiocarcinoma Cells via Down-Regulating CDKL3.

Authors - Jinduo Zhang
  • KD Validated
View Article