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  • Featured Product
  • KD/KO Validated

CDR2L Polyclonal antibody

CDR2L Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14563-1-AP

Synonyms

Paraneoplastic 62 kDa antigen, HUMPPA, Cerebellar degeneration-related protein 2-like

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CDR2L Polyclonal antibody (14563-1-AP)

    WB analysis of K-562 using 14563-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14563-1-AP (CDR2L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CDR2L Polyclonal antibody (14563-1-AP)

    WB analysis of SH-SY5Y using 14563-1-AP

    WB result of CDR2L antibody (14563-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDR2L transfected SH-SY5Y cells.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using CDR2L Polyclonal antibody (14563-1-AP)

    IP experiment of SH-SY5Y using 14563-1-AP

    IP result of anti-CDR2L (IP:14563-1-AP, 3ug; Detection:14563-1-AP 1:500) with SH-SY5Y cells lysate 2500ug.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using CDR2L Polyclonal antibody (14563-1-AP)

    IHC staining of human cerebellum using 14563-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using CDR2L Polyclonal antibody (14563-1-AP)

    IHC staining of human cerebellum using 14563-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDR2L Polyclonal antibody (14563-1-AP)

    IHC staining of mouse brain using 14563-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDR2L Polyclonal antibody (14563-1-AP)

    IHC staining of mouse brain using 14563-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDR2L Polyclonal antibody (14563-1-AP)

    IF Staining of HepG2 using 14563-1-AP

    Immunofluorescent analysis of HepG2 cells using 14563-1-AP (CDR2L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14563-1-AP targets CDR2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDR2L fusion protein Ag6127 相同性解析による交差性が予測される生物種
Full Name cerebellar degeneration-related protein 2-like
Calculated molecular weight 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC047534
Gene symbol CDR2L
Gene ID (NCBI) 30850
RRIDAB_2076740
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm. The predicted MW of this protein is 53 kDa.

Protocols

Product Specific Protocols
WB protocol for CDR2L antibody 14563-1-APDownload protocol
IHC protocol for CDR2L antibody 14563-1-APDownload protocol
IF protocol for CDR2L antibody 14563-1-APDownload protocol
IP protocol for CDR2L antibody 14563-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP, WB

Acta Neuropathol

Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.

Authors - Manja Schubert
View Article
humanWB,IHC

Cancer Immunol Immunother

Cerebellar degeneration-related proteins 2 and 2-like are present in ovarian cancer in patients with and without Yo antibodies.

Authors - Margrethe Raspotnig
View Article
humanWB,IF,IP

Ann Clin Transl Neurol

Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.

Authors - Ida Herdlevaer
View Article
human,ratWB,IHC

J Neuroimmunol

Expression of cerebellar degeneration-related proteins CDR2 and CDR2L in human and rat brain tissue.

Authors - Margrethe Raspotnig
View Article