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CDR2L Monoclonal antibody

CDR2L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2E12D10

Cat no : 66791-1-Ig

Synonyms

CDR2L, HUMPPA, Paraneoplastic 62 kDa antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using CDR2L Monoclonal antibody (66791-1-Ig)

    WB analysis of U-251 using 66791-1-Ig

    U-251 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CDR2L Monoclonal antibody (66791-1-Ig)

    WB analysis of rat brain using 66791-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CDR2L Monoclonal antibody (66791-1-Ig)

    WB analysis of HEK-293 using 66791-1-Ig

    WB result of CDR2L antibody (66791-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDR2L transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using CDR2L Monoclonal antibody (66791-1-Ig)

    WB analysis of HEK-293 using 66791-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CDR2L Monoclonal antibody (66791-1-Ig)

    WB analysis of K-562 using 66791-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CDR2L Monoclonal antibody (66791-1-Ig)

    WB analysis of human brain using 66791-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CDR2L Monoclonal antibody (66791-1-Ig)

    WB analysis of mouse brain using 66791-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using CDR2L Monoclonal antibody (66791-1-Ig)

    IHC staining of human cerebellum using 66791-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66791-1-Ig (CDR2L antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using CDR2L Monoclonal antibody (66791-1-Ig)

    IHC staining of human cerebellum using 66791-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66791-1-Ig (CDR2L antibody) at dilution of 1:400 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CDR2L Monoclonal antibody (66791-1-Ig)

    IF Staining of HEK-293 using 66791-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CDR2L antibody (66791-1-Ig, Clone: 2E12D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inU-251 cells, HEK-293 cells, K-562 cells, human brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IPSee 1 publications below

Product Information

66791-1-Ig targets CDR2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDR2L fusion protein Ag6139 相同性解析による交差性が予測される生物種
Full Name cerebellar degeneration-related protein 2-like
Calculated molecular weight 53 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC047534
Gene symbol CDR2L
Gene ID (NCBI) 30850
RRIDAB_2882135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for CDR2L antibody 66791-1-IgDownload protocol
IHC protocol for CDR2L antibody 66791-1-IgDownload protocol
IF protocol for CDR2L antibody 66791-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Ann Clin Transl Neurol

Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.

Authors - Ida Herdlevaer
View Article