Validation Data Gallery
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Tested Applications
| Positive WB detected in | U-251 cells, HEK-293 cells, K-562 cells, human brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IP | See 1 publications below |
Product Information
66791-1-Ig targets CDR2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CDR2L fusion protein Ag6139 相同性解析による交差性が予測される生物種 |
| Full Name | cerebellar degeneration-related protein 2-like |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | BC047534 |
| Gene Symbol | CDR2L |
| Gene ID (NCBI) | 30850 |
| RRID | AB_2882135 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q86X02 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDR2L antibody 66791-1-Ig | Download protocol |
| IHC protocol for CDR2L antibody 66791-1-Ig | Download protocol |
| IF protocol for CDR2L antibody 66791-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ann Clin Transl Neurol Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration. |