Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, NIH/3T3 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
Product Information
13184-1-AP targets CDSN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDSN fusion protein Ag3854 相同性解析による交差性が予測される生物種 |
| Full Name | corneodesmosin |
| Calculated molecular weight | 528 aa, 52 kDa |
| Observed molecular weight | 55-65 kDa |
| GenBank accession number | BC031993 |
| Gene Symbol | CDSN |
| Gene ID (NCBI) | 1041 |
| RRID | AB_11183052 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15517 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDSN, also named as S protein, is important for the epidermal barrier integrity. CDSN is a unique extracellular 52- to 56-kDa glycoprotein found in corneodesmosomes. Pathogenic mutations in CDSN have been linked in humans to genodermatoses affecting both the hair follicle and the epidermis. Inflammatory peeling skin syndrome is caused a novel mutation in CDSN.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDSN antibody 13184-1-AP | Download protocol |
| IHC protocol for CDSN antibody 13184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Sphingosine 1-Phosphate Receptor 2 Is Central to Maintaining Epidermal Barrier Homeostasis. | ||
iScience OBP2A regulates epidermal barrier function and protects against cytotoxic small hydrophobic molecules | ||
Exp Dermatol Acral peeling in Nagashima type palmo-plantar keratosis patients reveals the role of serine protease inhibitor B 7 in keratinocyte adhesion. | ||
Clin Cosmet Investig Dermatol Repeated short climatic change affects the epidermal differentiation program and leads to matrix remodeling in a human organotypic skin model. | ||
Heliyon Diagnostic, prognostic, and immunological roles of CDSN in ovarian cancer
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