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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDX1 Polyclonal antibody

CDX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21655-1-AP

Synonyms

Homeobox protein CDX-1, Homeobox protein CDX 1, Caudal-type homeobox protein 1, Caudal type homeobox protein 1, Caudal type homeobox 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDX1 Polyclonal antibody (21655-1-AP)

    WB analysis using 21655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CDX1 Polyclonal antibody (21655-1-AP)

    WB analysis using 21655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using CDX1 Polyclonal antibody (21655-1-AP)

    WB analysis of mouse colon using 21655-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse stomach tissue using CDX1 Polyclonal antibody (21655-1-AP)

    WB analysis of mouse stomach using 21655-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using CDX1 Polyclonal antibody (21655-1-AP)

    WB analysis of mouse small intestine using 21655-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using CDX1 Polyclonal antibody (21655-1-AP)

    WB analysis of mouse small intestine using 21655-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using CDX1 Polyclonal antibody (21655-1-AP)

    WB analysis of mouse small intestine using 21655-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Caco-2 cells using CDX1 Polyclonal antibody (21655-1-AP)

    IP experiment of Caco-2 using 21655-1-AP

    IP result of anti-CDX1 (IP:21655-1-AP, 4ug; Detection:21655-1-AP 1:1000) with Caco-2 cells lysate 1400 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDX1 Polyclonal antibody (21655-1-AP)

    IHC staining of human colon cancer using 21655-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21655-1-AP (CDX1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CDX1 Polyclonal antibody (21655-1-AP)

    IF Staining of Caco-2 using 21655-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX1 antibody (21655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inCaco-2 cells, mouse small intestine tissue, mouse stomach tissue, mouse colon tissue, rat colon tissue
Positive IP detected inCaco-2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21655-1-AP targets CDX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDX1 fusion protein Ag16376 相同性解析による交差性が予測される生物種
Full Name caudal type homeobox 1
Calculated molecular weight 265 aa, 28 kDa
Observed molecular weight 28 kDa, 35 kDa
GenBank accession numberBC096252
Gene symbol CDX1
Gene ID (NCBI) 1044
RRIDAB_2878901
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CDX1 antibody 21655-1-APDownload protocol
IHC protocol for CDX1 antibody 21655-1-APDownload protocol
IF protocol for CDX1 antibody 21655-1-APDownload protocol
IP protocol for CDX1 antibody 21655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols