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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDX2 Polyclonal antibody (22101-1-AP)

    WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDX2 Polyclonal antibody (22101-1-AP)

    WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDX2 Polyclonal antibody (22101-1-AP)

    WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CDX2 Polyclonal antibody (22101-1-AP)

    IHC staining of mouse small intestine using 22101-1-AP (same clone as 22101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CDX2 Polyclonal antibody (22101-1-AP)

    IHC staining of mouse small intestine using 22101-1-AP (same clone as 22101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CDX2 Polyclonal antibody (22101-1-AP)

    IHC staining of human appendicitis using 22101-1-AP (same clone as 22101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CDX2 Polyclonal antibody (22101-1-AP)

    IHC staining of human stomach cancer using 22101-1-AP (same clone as 22101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CDX2 Polyclonal antibody (22101-1-AP)

    IF Staining of Caco-2 using 22101-1-AP (same clone as 22101-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (22101-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

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CDX2 Polyclonal antibody, PBS Only

CDX2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22101-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22101-1-PBS targets CDX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDX2 fusion protein Ag17310 相同性解析による交差性が予測される生物種
Full Name caudal type homeobox 2
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight 33-40 kDa
GenBank accession numberBC014461
Gene Symbol CDX2
Gene ID (NCBI) 1045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99626
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.