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  • Featured Product
  • KD/KO Validated

CDX2 Monoclonal antibody, PBS Only (Capture)

CDX2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A4E6

Cat no : 60243-1-PBS

Synonyms

CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using CDX2 Monoclonal antibody (60243-1-Ig)

    WB analysis of COLO 320 using 60243-1-Ig (same clone as 60243-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW 1990 cells using CDX2 Monoclonal antibody (60243-1-Ig)

    WB analysis of SW 1990 using 60243-1-Ig (same clone as 60243-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using CDX2 Monoclonal antibody (60243-1-Ig)

    WB analysis of HT-29 using 60243-1-Ig (same clone as 60243-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig colon tissue using CDX2 Monoclonal antibody (60243-1-Ig)

    WB analysis of pig colon using 60243-1-Ig (same clone as 60243-1-PBS)

    pig colon tissue were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CDX2 Monoclonal antibody (60243-1-Ig)

    IF Staining of Caco-2 using 60243-1-Ig (same clone as 60243-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (60243-1-Ig, Clone: 5A4E6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50121-1

    Cytometric bead array standard curve of MP50121-1

    Cytometric bead array standard curve of MP50121-1, CDX2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60243-1-PBS. Detection antibody: 60243-3-PBS. Standard:Ag17644. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60243-1-PBS targets CDX2 as part of a matched antibody pair:

MP50121-1: 60243-1-PBS capture and 60243-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDX2 fusion protein Ag17644 相同性解析による交差性が予測される生物種
Full Name caudal type homeobox 2
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight 33-40 kDa
GenBank accession numberBC014461
Gene symbol CDX2
Gene ID (NCBI) 1045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.