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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDX2 Recombinant antibody, PBS Only

CDX2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3J1

Cat no : 82659-1-PBS

Synonyms

CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDX2 Recombinant antibody (82659-1-RR)

    WB analysis using 82659-1-RR (same clone as 82659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using CDX2 Recombinant antibody (82659-1-RR)

    WB analysis of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using CDX2 Recombinant antibody (82659-1-RR)

    IHC staining of rat colon using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDX2 Recombinant antibody (82659-1-RR)

    IHC staining of human colon cancer using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDX2 Recombinant antibody (82659-1-RR)

    IHC staining of human colon cancer using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CDX2 Recombinant antibody (82659-1-RR)

    IF Staining of Caco-2 using 82659-1-RR (same clone as 82659-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (82659-1-RR, Clone: 3J1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82659-1-RR

    Affinity and Kinetic Characterization of 82659-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82659-1-RR against Human CDX2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82659-1-PBS targets CDX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDX2 fusion protein Ag17310 相同性解析による交差性が予測される生物種
Full Name caudal type homeobox 2
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight 33-40 kDa
GenBank accession numberBC014461
Gene symbol CDX2
Gene ID (NCBI) 1045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.