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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEA/CD66e Monoclonal antibody (68377-1-Ig)

    WB analysis using 68377-1-Ig (same clone as 68377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CEA/CD66e Monoclonal antibody (68377-1-Ig)

    WB analysis using 68377-1-Ig (same clone as 68377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CEA/CD66e Monoclonal antibody (68377-1-Ig)

    IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CEA/CD66e Monoclonal antibody (68377-1-Ig)

    IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CEA/CD66e Monoclonal antibody (68377-1-Ig)

    IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CEA/CD66e Monoclonal antibody (68377-1-Ig)

    IHC staining of human stomach cancer using 68377-1-Ig (same clone as 68377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CEA/CD66e Monoclonal antibody (68377-1-Ig)

    IHC staining of human stomach cancer using 68377-1-Ig (same clone as 68377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CEA/CD66e Monoclonal antibody (68377-1-Ig)

    IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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CEA/CD66e Monoclonal antibody, PBS Only

CEA/CD66e Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68377-1-PBS
Clone No.2B8H6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68377-1-PBS targets CEA/CD66e in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Calculated molecular weight 77 kDa
Observed molecular weight180-200 kDa
GenBank accession numberBC034671
Gene Symbol CEA
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06731
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.