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  • Featured Product
  • KD/KO Validated

CEA/CD66e Polyclonal antibody, PBS Only

CEA/CD66e Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10421-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEA/CD66e Polyclonal antibody (10421-1-AP)

    WB analysis using 10421-1-AP (same clone as 10421-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10421-1-AP (CEA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CEA/CD66e Polyclonal antibody (10421-1-AP)

    WB analysis of MCF-7 using 10421-1-AP (same clone as 10421-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10421-1-AP (CEA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CEA/CD66e Polyclonal antibody (10421-1-AP)

    IHC staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CEA/CD66e Polyclonal antibody (10421-1-AP)

    IHC staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CEA/CD66e Polyclonal antibody (10421-1-AP)

    IHC staining of human lung cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10421-1-AP (CEA antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CEA/CD66e Polyclonal antibody (10421-1-AP)

    IHC staining of human lung cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10421-1-AP (CEA antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CEA/CD66e Polyclonal antibody (10421-1-AP)

    IHC staining of human stomach cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10421-1-AP (CEA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CEA/CD66e Polyclonal antibody (10421-1-AP)

    IHC staining of human stomach cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10421-1-AP (CEA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CEA/CD66e Polyclonal antibody (10421-1-AP)

    IHC staining of human appendicitis using 10421-1-AP (same clone as 10421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CEA/CD66e Polyclonal antibody (10421-1-AP)

    IF Staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CEA/CD66e Polyclonal antibody (10421-1-AP)

    IF Staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10421-1-PBS targets CEA/CD66e in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEA/CD66e fusion protein Ag0672 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Calculated molecular weight 77 kDa
Observed molecular weight180-200 kDa, 77 kDa
GenBank accession numberBC034671
Gene symbol CEA
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.