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Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CEACAM1/CD66a Rabbit Recombinant Antibo (98185-1-RR)

    FC experiment of mouse splenocytes using 98185-1-RR (same clone as 98185-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CEACAM1/CD66a Rabbit Recombinant Antibody (98185-1-RR, Clone: 241856H5) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD45R. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98185-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CEACAM1/CD66a Rabbit Recombinant Antibo (98185-1-RR)

    FC experiment of mouse splenocytes using 98185-1-RR (same clone as 98185-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CEACAM1/CD66a Rabbit Recombinant Antibody (98185-1-RR, Clone: 241856H5) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD45R. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98185-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98185-1-RR

    Affinity and Kinetic Characterization of 98185-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98185-1-RR against Mouse CEACAM1/CD66a were performed. The affinity constant is below 1 pM.

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Anti-Mouse CEACAM1/CD66a Rabbit Recombinant Antibody, PBS Only

CEACAM1/CD66a Uni-rAbTM Recombinant Antibody for FC
Cat No. 98185-1-PBS
Clone No.241856H5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1, Bgp

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98185-1-PBS targets CEACAM1/CD66a in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 1
Calculated molecular weight57 kDa
Gene Symbol Ceacam1
Gene ID (NCBI) 26365
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31809-1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).