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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using CEACAM1/CD66a Polyclonal antibody (30347-1-AP)

    IHC staining of human colon using 30347-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30347-1-AP (CEACAM1/CD66a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CEACAM1/CD66a Polyclonal antibody (30347-1-AP)

    IHC staining of human colon using 30347-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30347-1-AP (CEACAM1/CD66a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CEACAM1/CD66a Polyclonal antibody

CEACAM1/CD66a Polyclonal Antibody for IHC, ELISA
Cat No. 30347-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

CEACAM1, Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGPI, BGP-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30347-1-AP targets CEACAM1/CD66a in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Calculated molecular weight 526 aa, 58 kDa
GenBank accession numberBC014473
Gene Symbol CEACAM1
Gene ID (NCBI) 634
RRIDAB_3086298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13688
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

Protocols

Product Specific Protocols
IHC protocol for CEACAM1/CD66a antibody 30347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols