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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEACAM1/CD66a Polyclonal antibody

CEACAM1/CD66a Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 30347-1-AP

Synonyms

CEACAM1, Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGPI, BGP-1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using CEACAM1/CD66a Polyclonal antibody (30347-1-AP)

    IHC staining of human colon using 30347-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30347-1-AP (CEACAM1/CD66a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CEACAM1/CD66a Polyclonal antibody (30347-1-AP)

    IHC staining of human colon using 30347-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30347-1-AP (CEACAM1/CD66a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30347-1-AP targets CEACAM1/CD66a in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Calculated molecular weight 526 aa, 58 kDa
GenBank accession numberBC014473
Gene symbol CEACAM1
Gene ID (NCBI) 634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

Protocols

Product Specific Protocols
IHC protocol for CEACAM1/CD66a antibody 30347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols