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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CEA/CD66e Recombinant antibody (83653-5-RR)

    WB analysis of MCF-7 using 83653-5-RR (same clone as 83653-5-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human saliva using CEA/CD66e Recombinant antibody (83653-5-RR)

    WB analysis of human saliva using 83653-5-RR (same clone as 83653-5-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CEA/CD66e Recombinant antibody (83653-5-RR)

    IHC staining of human ovary cancer using 83653-5-RR (same clone as 83653-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CEA/CD66e Recombinant antibody (83653-5-RR)

    IHC staining of human ovary cancer using 83653-5-RR (same clone as 83653-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CEA/CD66e Recombinant antibody (83653-5-RR)

    IF Staining of HT-29 using 83653-5-RR (same clone as 83653-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CEA antibody (83653-5-RR, Clone: 240666B12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83653-5-RR

    Affinity and Kinetic Characterization of 83653-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83653-5-RR against Human CEA were performed. The affinity constant is 0.734 nM.

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CEA/CD66e Recombinant antibody, PBS Only

CEA/CD66e Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83653-5-PBS
Clone No.240666B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83653-5-PBS targets CEA/CD66e in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Calculated molecular weight 77 kDa
Observed molecular weight200 kDa
GenBank accession numberNM_004363
Gene Symbol CEA
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06731-1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis. This antibody is raised against 35-685 aa of human CEA/CD66e and can cross-react with CEACAM1/CD66a and CEACAM3/CD66d.