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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEACAM7 Polyclonal antibody

CEACAM7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24626-1-AP

Synonyms

Carcinoembryonic antigen CGM2, CEA, CEACAM7, CGM2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using CEACAM7 Polyclonal antibody (24626-1-AP)

    WB analysis of mouse colon using 24626-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24626-1-AP (CEACAM7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using CEACAM7 Polyclonal antibody (24626-1-AP)

    IHC staining of human colon using 24626-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24626-1-AP (CEACAM7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CEACAM7 Polyclonal antibody (24626-1-AP)

    IHC staining of human colon using 24626-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24626-1-AP (CEACAM7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24626-1-AP targets CEACAM7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEACAM7 fusion protein Ag18579 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 7
Calculated molecular weight 265 aa, 29 kDa
Observed molecular weight58 kDa
GenBank accession numberBC121132
Gene symbol CEACAM7
Gene ID (NCBI) 1087
RRIDAB_3085742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEACAM7, also known as CGM2, belongs to the carcinoembryonic antigen (CEA) family. CEACAM7 contains three domains: an N-terminal IgV domain, a single IgC2 domain, and a cell-surface GPI anchor domain. CEACAM7 achieves cell adhesion through dimerization of the N-terminal IgV domain. CEACAM7 is expressed on highly differentiated epithelial cells of the colon and rectum and on the epithelial cells within the ducts of the pancreas (PMID: 26323304). CEACAM7 has 2 isoforms with the molecular mass of 19 and 29 kDa. The observed MW of CEACAM7 is 58 kDa, which is consistent with the description in the literature (PMID: 36828119).

Protocols

Product Specific Protocols
WB protocol for CEACAM7 antibody 24626-1-APDownload protocol
IHC protocol for CEACAM7 antibody 24626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols