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CEBP Alpha/CEBPA Polyclonal antibody

CEBP Alpha/CEBPA Polyclonal Antibody for WB, IP, ELISA
Cat No. 29388-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

CEBPA, CEBP alpha, CEBP, CCAAT/enhancer-binding protein alpha, C/EBPα

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 1500 μg/ml
20 μL, 1500 μg/ml
150 μL, 1500 μg/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inBGC-823 cells, LNCaP cells, THP-1 cells, U-937 cells, K-562 cells, L02 cells, HL-60 cells
Positive IP detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29388-1-AP targets CEBP Alpha/CEBPA in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEBP Alpha/CEBPA fusion protein Ag29947 相同性解析による交差性が予測される生物種
Full Name CCAAT/enhancer binding protein (C/EBP), alpha
Calculated molecular weight 38 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberNM_004364
Gene Symbol CEBPA
Gene ID (NCBI) 1050
RRIDAB_3086127
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49715
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc. The calcualted molecular weight of CEBPA is 38 kDa, but modified CEBPA is about 42 kDa (PMID: 19623175).

Protocols

Product Specific Protocols
WB protocol for CEBP Alpha/CEBPA antibody 29388-1-APDownload protocol
IP protocol for CEBP Alpha/CEBPA antibody 29388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Front Pharmacol

Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism

Authors - Tian Li
WB

J Virol

Dengue virus NS1 leads to downregulation of HNF4 alpha in liver cells resulting in a decrease in coagulation factors I, V, X, and XIII, contributing to coagulopathy

Authors - Sandeepan Das
mouseWB

Pharmaceuticals (Basel)

Potential Anti-Obesity Effect of Hazel Leaf Extract in Mice and Network Pharmacology of Selected Polyphenols

Authors - Jiarui Zhao
humanWB,IHC

Sci Rep

A comprehensive analysis of CEBPA on prognosis and function in uterine corpus endometrial carcinoma

Authors - Jiaxing Wang
humanWB

Genes Dis

MAFB-mediated CEBPA regulated human urothelium growth through Wnt/β-catenin signaling pathway

Authors - Zhenmin Liu
ratWB

Mol Med

Ginsenoside Rb1 affects mitochondrial Ca2+ transport and inhibits fat deposition and fibrosis by regulating the wnt signaling pathway to treat rotator cuff tears via docking with SFRP1

Authors - Yuesong Yin
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