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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR)

    IF Staining of HeLa using 84668-4-RR (same clone as 84668-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CEBP Alpha/CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR)

    IF Staining of HepG2 using 84668-4-RR (same clone as 84668-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBP Alpha/CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR)

    IF Staining of HepG2 using 84668-4-RR (same clone as 84668-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBP Alpha/CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR)

    FC experiment of HeLa using 84668-4-RR (same clone as 84668-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR, Clone:242101C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR)

    FC experiment of HepG2 using 84668-4-RR (same clone as 84668-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR, Clone:242101C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84668-4-RR

    Affinity and Kinetic Characterization of 84668-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84668-4-RR against Human CEBP Alpha/CEBPA were performed. The affinity constant is 1.85 nM.

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CEBP Alpha/CEBPA Recombinant antibody, PBS Only

CEBP Alpha/CEBPA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84668-4-PBS
Clone No.242101C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CEBP alpha, CEBPA, CEBP, CCAAT/enhancer-binding protein alpha, C/EBPα

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84668-4-PBS targets CEBP Alpha/CEBPA in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CEBP Alpha/CEBPA fusion protein Ag29947 相同性解析による交差性が予測される生物種
Full Name CCAAT/enhancer binding protein (C/EBP), alpha
Calculated molecular weight 38 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberNM_004364
Gene Symbol CEBPA
Gene ID (NCBI) 1050
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49715
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc.