CEBPB Recombinant antibody
CEBPB Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240836G6
Cat no : 83791-6-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, A549 cells, U-87 MG cells, MKN-45 cells, MDA-MB-231 cells |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83791-6-RR is CEBPB Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | CEBPB fusion protein Ag20073 相同性解析による交差性が予測される生物種 |
Full Name | CCAAT/enhancer binding protein (C/EBP), beta |
Calculated molecular weight | 345 aa, 36 kDa |
Observed molecular weight | 42 kDa, 46 kDa |
GenBank accession number | BC007538 |
Gene symbol | CEBPB |
Gene ID (NCBI) | 1051 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298).
Protocols
Product Specific Protocols | |
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WB protocol for CEBPB antibody 83791-6-RR | Download protocol |
IF protocol for CEBPB antibody 83791-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |