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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CEBPZ Polyclonal antibody (25612-1-AP)

    WB analysis of HeLa using 25612-1-AP (same clone as 25612-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CEBPZ Polyclonal antibody (25612-1-AP)

    WB analysis using 25612-1-AP (same clone as 25612-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CEBPZ Polyclonal antibody (25612-1-AP)

    WB analysis using 25612-1-AP (same clone as 25612-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CEBPZ Polyclonal antibody (25612-1-AP)

    WB analysis of mouse testis using 25612-1-AP (same clone as 25612-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CEBPZ Polyclonal antibody (25612-1-AP)

    WB analysis of mouse testis using 25612-1-AP (same clone as 25612-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CEBPZ Polyclonal antibody (25612-1-AP)

    WB analysis of mouse testis using 25612-1-AP (same clone as 25612-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CEBPZ Polyclonal antibody (25612-1-AP)

    WB analysis of mouse testis using 25612-1-AP (same clone as 25612-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEBPZ Polyclonal antibody (25612-1-AP)

    IF Staining of HeLa using 25612-1-AP (same clone as 25612-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 25612-1-AP (CEBPZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

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CEBPZ Polyclonal antibody, PBS Only

CEBPZ Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 25612-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25612-1-PBS targets CEBPZ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEBPZ fusion protein Ag11003 相同性解析による交差性が予測される生物種
Full Name CCAAT/enhancer binding protein (C/EBP), zeta
Calculated molecular weight 1054 aa, 121 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC034475
Gene Symbol CEBPZ
Gene ID (NCBI) 10153
RRIDAB_2880160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03701
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CEBPZ (CCAAT/enhancer binding protein zeta) belongs to the CBF/Mak21 family, it plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner.