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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CELSR2 Recombinant antibody (83115-1-RR)

    IF Staining of SH-SY5Y using 83115-1-RR (same clone as 83115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CELSR2 antibody (83115-1-RR, Clone: 230319H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83115-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CELSR2 Recombinant antibody (83115-1-RR)

    FC experiment of HeLa using 83115-1-RR (same clone as 83115-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CELSR2 Recombinant antibody (83115-1-RR, Clone:230319H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83115-1-PBS in a different storage buffer formulation.

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CELSR2 Recombinant antibody, PBS Only

CELSR2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83115-1-PBS
Clone No.230319H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EGFL2, EGF like protein 2, CDHF10, Cadherin family member 10, Cadherin EGF LAG seven-pass G-type receptor 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83115-1-PBS targets CELSR2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
Calculated molecular weight 318 kDa
GenBank accession numberNM_001408
Gene Symbol CELSR2
Gene ID (NCBI) 1952
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9HCU4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CELSR2, also named CDHF10, EGFL2, KIAA0279, and MEGF3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is a receptor that may be important in cell/cell signaling during nervous system formation.