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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CENPB Polyclonal antibody (26738-1-AP)

    WB analysis using 26738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26738-1-AP (CENPB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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CENPB Polyclonal antibody

CENPB Polyclonal Antibody for WB, ELISA
Cat No. 26738-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CENP B, CENPB, Centromere protein B, centromere protein B, 80kDa, Major centromere autoantigen B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26738-1-AP targets CENPB in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPB fusion protein Ag24967 相同性解析による交差性が予測される生物種
Full Name centromere protein B, 80kDa
Calculated molecular weight 65 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC053847
Gene Symbol CENPB
Gene ID (NCBI) 1059
RRIDAB_3085899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07199
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Centromere protein B (CENPB), also known as major centromere autoantigen B, is an autoantigen protein of the cell nucleus. In mammalian centromeres CENPB plays a critical role in the organization of chromatinstructure, but in the absence of CENPB some chromosomes use other redundant proteins to accomplish mitosis (PMID: 14963831). CENPB plays an important role in cell cycle regulation. CENPB is highly expressed in cancer cells helping in high rate proliferation. This antibody detects CENPB protein with a molecular weight of 80 kDa (PMID: 34547289, PMID: 29273057).

Protocols

Product Specific Protocols
WB protocol for CENPB antibody 26738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols