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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CENPC Polyclonal antibody (14250-1-AP)

    WB analysis of HeLa using 14250-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14250-1-AP (CENPC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CENPC Polyclonal antibody (14250-1-AP)

    WB analysis of Jurkat using 14250-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14250-1-AP (CENPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CENPC Polyclonal antibody (14250-1-AP)

    WB analysis of A431 using 14250-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 14250-1-AP (CENPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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CENPC Polyclonal antibody

CENPC Polyclonal Antibody for WB, ELISA
Cat No. 14250-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CENP C, CENP C 1, CENPC, CENPC1, Centromere autoantigen C, centromere protein C 1, hcp 4, ICEN7, MIF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A431 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14250-1-AP targets CENPC in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPC fusion protein Ag5523 相同性解析による交差性が予測される生物種
Full Name centromere protein C 1
Calculated molecular weight 107 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC041117
Gene Symbol CENPC
Gene ID (NCBI) 1060
RRIDAB_10642008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03188
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CENPC antibody 14250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols