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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CENPF Polyclonal antibody (28568-1-AP)

    WB analysis of HeLa using 28568-1-AP (same clone as 28568-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 28568-1-AP (CENPF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CENPF Polyclonal antibody (28568-1-AP)

    WB analysis using 28568-1-AP (same clone as 28568-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28568-1-AP (CENPF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of rat small intestine using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of rat small intestine using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of human lung cancer using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of human lung cancer using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of rat testis using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of mouse kidney using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of mouse liver using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of human pancreas cancer using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of mouse small intestine using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using CENPF Polyclonal antibody (28568-1-AP)

    IHC staining of rat skin using 28568-1-AP (same clone as 28568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CENPF Polyclonal antibody (28568-1-AP)

    IF Staining of HeLa using 28568-1-AP (same clone as 28568-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (28568-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CENPF Polyclonal antibody (28568-1-AP)

    IF Staining of HeLa using 28568-1-AP (same clone as 28568-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (28568-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28568-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CENPF Polyclonal antibody, PBS Only

CENPF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28568-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PRO1779, CENP-F, CENF

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28568-1-PBS targets CENPF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPF fusion protein Ag29665 相同性解析による交差性が予測される生物種
Full Name centromere protein F, 350/400ka (mitosin)
Calculated molecular weight 368 kDa
Observed molecular weight350 kDa
GenBank accession numberNM_016343
Gene Symbol CENPF
Gene ID (NCBI) 1063
RRIDAB_2918176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49454
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).