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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CENPF Recombinant antibody (84865-4-RR)

    IF Staining of HeLa using 84865-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84865-4-RR

    Affinity and Kinetic Characterization of 84865-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84865-4-RR against Human CENPF were performed. The affinity constant is 11.1 nM.

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CENPF Recombinant antibody

CENPF Recombinant Antibody for IF/ICC, ELISA
Cat No. 84865-4-RR
Clone No.242277G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

PRO1779, CENP-F, CENF, 242277G9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84865-4-RR targets CENPF in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CENPF fusion protein Ag29665 相同性解析による交差性が予測される生物種
Full Name centromere protein F, 350/400ka (mitosin)
Calculated molecular weight 368 kDa
GenBank accession numberNM_016343
Gene Symbol CENPF
Gene ID (NCBI) 1063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49454
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

Protocols

Product Specific Protocols
IF protocol for CENPF antibody 84865-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols